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LIPSI Microscope

LS
05/09/2022
Partager
High-speed, flexible, automated well plate acquisition, data visualization and analysis.
Nikon Europe has developed, in collaboration with key players in the field Prior scientific and Life Imaging Services, a fully incubated, high content screening platform that enables high speed acquisition for up to 20 well plates. Together with a analysis tools, Nikon enables you to get results from live cells in real time.
LIPSI system

Principle

Hight Content Screening HCS

Recent advances in hardware and software innovation for automated image analysis now permits researchers to rapidly screen and analyse hundreds of thousands of images.

High-content screening (HCS), which combines automated fluorescence microscopy with quantitative image analysis, allows the acquisition of unbiased multiparametric data at the single-cell level. This approach has been used to address diverse biological questions, and identify quantitative phenotypes of varying complexity, in numerous different model systems.

In practice, one may acquire, for example, a 96 well plate by scanning the wells faster with a large field of view (up to 25mm). In live imaging, the well plates are hosted in the dedicated opaque chamber to preserve the cells. Without any temperature shock during transfer under a temperature-controlled environment, CO2 and humidity/hygrometry, we can follow the growing cells.

In addition, due to the combination of fluorescence imaging and bright field illumination, the application in immuno fluorescence and histology find a real interest for a large number slice screening.

 

Configuration

LIPSI utilises all the functionality of the Eclipse Ti2 inverted microscope, including its 25mm field of view and capacity of high speed imaging, while offering modularity and expandability. LIPSI can easily be upgraded with different confocal modalities and accessories to ensure that your platform can evolve with your research needs.

The current configuration of LIPSI is equipped with an LSM microscopy for the high-speed and resolutive confocal A1R HD25 coupled with wide field color camera imaging Nikon DS-Fi3 colour for the histiological acquisitions. Fully automated and motorised from the plate handelling to the stage, we can acquire, visualize and analyse in the real time the data.

Objectives

Magnification    Type    Immersion N.A WD  
4x Plan Fluor Dry 0.2 20  
10x CFI Plan Fluor dry 0.45 4  
20x CFI Apo LWD Lambda S  dry 0.95 0.99 – 0.9 DIC
40x CFI Plan Fluor dry 0.95 0.21 -
40x  CFI S Fluor Water 1.15 0.61 – 0.59 DIC 
60x CFI Plan Apo VC 60XC  Water 1.2  0.31 – 0.28 DIC

 

Filters

In widefield illumination Excitation filter   DM Emission filter  
LED-DAPI/FITC/TRICT/Cy5-B 352-404/461-88/543-66/626-44 414-50/500-30/580-611/660-800 414-50/500-30/580-611/660-800
LED-CFP/YFP/mCh-A 426-50/498-520/567-589 464-89/532-54/603-800 464-86/532-54/603-800

 

In Confocal  illumination DM1 DM2 Emission filter  
  405/488/561/640 495LP 450/50
  BS 20/80 560LP 525/50
    640LP 595-50/700-75

 

Associated Devices

Illumination Detector                    Software Z motor T° & CO2  Motorization 
EPI Illumination : Spectra X Color camera DS-Fi3 NIS-Elements Ti2 Z Motor Special design, Life Imaging Service, LIS  Prior Robot
Confocal LU-N4: 405/488/561/640 nm lasers built-in AOTF

2 GaAsP PMTs + 2 Multi-Alkali PMTs

Spectral detector unit with 32 channels

    RaspBerry Camera, LIS 

 

Techniques

  • Brightfield
  • Fluorescence
  • Live Imaging 4D5D : Time lapse acquisition in 2D, 3D, multi-position and multicolors
  • Fast Scan Microscopy (LSM)
  • Screening

Applications

  • Cell imaging, drugs testing
  • Life Imaging for organoids
  • Fish development
  • Histology scanning