3D Deconvolution Microscope

04/12/2022

Nikon's new Ni Series microscopes were developed to address the need for a microscope that is highly versatile, combining system expandability and superior optical performance in an easy-to-use system. The Ni-U can be configured in a multitude of ways depending on the requirements of the user. Its adaptability makes it suitable for many applications, from clinical examination to research, and features motorized accessories that include nosepiece, fluorescence attachment, and shutter.

Fluorescence Microscopy

The absorption and subsequent re-radiation of light by organic and inorganic specimens is typically the result of well-established physical phenomena described as being either fluorescence or phosphorescence. The emission of light through the fluorescence process is nearly simultaneous with the absorption of the excitation light due to a relatively short time delay between photon absorption and emission, ranging usually less than a microsecond in duration. When emission persists longer after the excitation light has been extinguished, the phenomenon is referred to as phosphorescence. More..

Deconvolution

Deconvolution is a computational method that treats the image as an estimate of the true specimen intensity and using an expression for the point spread function performs the mathematical inverse of the imaging process to obtain an improved estimate of the image intensity. MicroscopyU

Configuration

Objectives :

Magnification	Type	Immersion	N.A	WD	DIC
100x	Plan Apo VC	Oil	1.4	0.13	Yes

Filters :

Name	EX	BS	EM
DAPI	AT350/50x	T400lp	ET460/50m
CFP	ET436/20x	T455lp	ET480/40m
GFP	ET470/40x	T495lpxr	ET525/50m
Cy3	ET545/25x	T565lpxr	ET605/70m
mCh	ET560/40x	T585lpxr	ET630/75m
Cy5	ET620/60x	T660lpxr	ET700/75m

Associated Devices :

Illumination	Detector	Software	Z motor	T°C & CO2
Intensilight	Photometric CCD Camera CoolSNAP HQ2	Metamorph	NiE Z motor	NO

Techniques

Brightfield
Epi-fluorescence
Deconvolution

Applications

Immunofluorescence fixed samples