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3D Deconvolution Microscope

LS
04/12/2022
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Nikon's new Ni Series microscopes were developed to address the need for a microscope that is highly versatile, combining system expandability and superior optical performance in an easy-to-use system. The Ni-U can be configured in a multitude of ways depending on the requirements of the user. Its adaptability makes it suitable for many applications, from clinical examination to research, and features motorized accessories that include nosepiece, fluorescence attachment, and shutter.
3Ddec NiE_1

Fluorescence Microscopy

The absorption and subsequent re-radiation of light by organic and inorganic specimens is typically the result of well-established physical phenomena described as being either fluorescence or phosphorescence. The emission of light through the fluorescence process is nearly simultaneous with the absorption of the excitation light due to a relatively short time delay between photon absorption and emission, ranging usually less than a microsecond in duration. When emission persists longer after the excitation light has been extinguished, the phenomenon is referred to as phosphorescence. More..

Deconvolution

Deconvolution is a computational method that treats the image as an estimate of the true specimen intensity and using an expression for the point spread function performs the mathematical inverse of the imaging process to obtain an improved estimate of the image intensity. MicroscopyU

3Ddec_KSchauer
melanosomes
hela-cells
actin-cytoskel
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Configuration

Objectives :

Magnification    Type    Immersion N.A WD DIC
100x Plan Apo VC Oil 1.4 0.13 Yes

Filters : 

Name EX BS EM
DAPI AT350/50x T400lp ET460/50m
CFP ET436/20x T455lp ET480/40m
GFP ET470/40x T495lpxr ET525/50m
Cy3 ET545/25x T565lpxr ET605/70m
mCh ET560/40x T585lpxr ET630/75m
Cy5 ET620/60x T660lpxr ET700/75m

Associated Devices : 

Illumination Detector Software Z motor T°C & CO2 
Intensilight Photometric CCD Camera CoolSNAP HQ2 Metamorph  NiE Z motor  NO

Techniques

 

Applications

  • Immunofluorescence fixed samples