Video 4D5D Microscope

The Ti2's exceptionally stable, drift-free platform is designed to meet the demands of super-resolution imaging while its unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications.Furthermore, the Ti2's unique, intelligent functions guide users through imaging workflows by gathering data from internal sensors, eliminating the possibility of user errors. In addition, the status of each sensor is automatically recorded during acquisition, providing quality control for imaging experiments and enhancing data reproducibility.
Principle
The Automatic Microscope
Motorized microscope components and accessories enable the investigator to automate live-cell image acquisition and are particularly useful for time-lapse experiments that range in timescale intervals from milliseconds to tens or hundreds of minutes. A wide variety of aftermarket auxiliary components, such as electromechanical shutters, microprocessor-controlled filter changers (filter wheels), motorized stages, and axial focus control mechanisms can be retrofitted to a research grade microscope and interactively controlled by a companion workstation computer using commercially available image acquisition software packages. However, it should be noted that assembling a fully automated and optimized multi-dimension optical imaging system is an extremely complex task. More...
Configuration
Objectives :
Magnification |
Type |
Immersion |
N.A |
WD |
|
10x |
Plan Fluor |
Dry |
0.3 |
16 |
Ph |
20x |
Plan Fluor |
Dry |
0.75 |
1 |
Ph |
20x |
Apo VC |
Dry |
0.8 |
0.8 |
DIC |
40x |
S Fluor ELWD |
Dry |
0.6 |
3.6-2.8 |
Ph |
40x |
Plan Fluor |
Oil |
1.3 |
0.24 |
DIC |
60x |
Plan Apo VC |
Oil |
1.4 |
0.13 |
DIC |
Filters :
Name |
EX |
BS |
EM |
AT350/50x |
T400lp |
ET460/50m |
|
ET470/40x |
T495lpxr |
ET525/50m |
|
ET545/25x |
T565lpxr |
ET605/70m |
|
ET560/40x |
T585lpxr |
ET630/75m |
|
ET620/60x |
T660lpxr |
ET700/75m |
Associated Devices :
Illumination | Detector | Software | Z motor | T°C & CO2 |
Spectra X LED |
Flash 4.0 V2 sCMOS Hamamatsu camera Kinetix sCMOS Photometrics camera |
NIS-Elements | NiE Z motor |
Themobox & Stage heater STXF-TIZWX-SET, Tokai Hit Co. |
Techniques
- Fluorescence
- Live Imaging (2D, 3D)
Applications
- Live Cell Imaging