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Video 4D5D Microscope

LS
04/12/2022
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The ECLIPSE Ti2 inverted microscope delivers an unparalleled 25mm field of view (FOV) that revolutionizes the way you see. With this incredible FOV, the Ti2 maximizes the sensor area of large-format CMOS cameras without making compromises, and significantly improves data throughput.
video4D5D Ti2_1

The Ti2's exceptionally stable, drift-free platform is designed to meet the demands of super-resolution imaging while its unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications.Furthermore, the Ti2's unique, intelligent functions guide users through imaging workflows by gathering data from internal sensors, eliminating the possibility of user errors. In addition, the status of each sensor is automatically recorded during acquisition, providing quality control for imaging experiments and enhancing data reproducibility.

Principle

The Automatic Microscope

Motorized microscope components and accessories enable the investigator to automate live-cell image acquisition and are particularly useful for time-lapse experiments that range in timescale intervals from milliseconds to tens or hundreds of minutes. A wide variety of aftermarket auxiliary components, such as electromechanical shutters, microprocessor-controlled filter changers (filter wheels), motorized stages, and axial focus control mechanisms can be retrofitted to a research grade microscope and interactively controlled by a companion workstation computer using commercially available image acquisition software packages. However, it should be noted that assembling a fully automated and optimized multi-dimension optical imaging system is an extremely complex task. More...

Configuration

Objectives :

Magnification

Type

Immersion

N.A

WD

 

10x

Plan Fluor

Dry

0.3

16

Ph

20x

Plan Fluor

Dry

0.75

1

Ph

20x

Apo VC

Dry

0.8

0.8

DIC

40x

S Fluor ELWD

Dry

0.6

3.6-2.8

Ph

40x

Plan Fluor

Oil

1.3

0.24

DIC

60x

Plan Apo VC

Oil

1.4

0.13

DIC

Filters : 

Name

EX

BS

EM

DAPI

AT350/50x

T400lp

ET460/50m

GFP

ET470/40x

T495lpxr

ET525/50m

Cy3

ET545/25x

T565lpxr

ET605/70m

mCh

ET560/40x

T585lpxr

ET630/75m

Cy5

ET620/60x

T660lpxr

ET700/75m

Associated Devices : 

Illumination Detector Software Z motor T°C & CO2 
Spectra X LED  Flash 4.0 V2  sCMOS Hamamatsu camera NIS-Elements  NiE Z motor 

Themobox & Stage heater STXF-TIZWX-SET, Tokai Hit Co.

Techniques

  • Fluorescence
  • Live Imaging (2D, 3D)

Applications

  • Live Cell Imaging
4D5D Videomicroscope