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Video 4D ECLIPSE Ti2 Inverted Microscope

LS
07/04/2017
Partager
Live-Cell Imaging
An increasing number of investigations are using live-cell imaging techniques to provide critical insight into the fundamental nature of cellular and tissue function, especially due to the rapid advances that are currently being witnessed in fluorescent protein and synthetic fluorophore technology.
eclipse-ti-e

Equipment Configuration

Illumination

Detectors

  • Camera : CMOS Flash4 V0.2 camera (Hamamatsu)

Objectives

10x

CFI Plan Fluor DL

NA 0.3

WD 15.2

Ph1

Dry

20x

CFI S Plan Fluor ELWD

NA 0.45

WD 8.2-6.9

Ph2

Dry

20x

CFI Plan APO VC

NA 0.75

WD 1

DIC

Dry

40x

CFI S Plan Fluor ELWD

NA 0.6

WD 3.6-2.8

Ph2

Oil

40x

CFI Plan Fluor

NA 1.4

WD 0.2

DIC

Oil

60x

CFI Plan Apo VC

NA 1.4

WD 0.13

DIC

Oil

multi-well-pink
multi-well-blue
multi-well-green

Bloc Filters

Associated Devices

Techniques

  • Brightfield
  • Epi-fluorescence
  • Phase contrast
  • DIC
  • Time lapse acquisition in 2D, 3D, multiposition and multicolors

Applications

  • Cell Biology

Principle

The Automatic Microscope

Motorized microscope components and accessories enable the investigator to automate live-cell image acquisition and are particularly useful for time-lapse experiments that range in timescale intervals from milliseconds to tens or hundreds of minutes. A wide variety of aftermarket auxiliary components, such as electromechanical shutters, microprocessor-controlled filter changers (filter wheels), motorized stages, and axial focus control mechanisms can be retrofitted to a research grade microscope and interactively controlled by a companion workstation computer using commercially available image acquisition software packages. However, it should be noted that assembling a fully automated and optimized multi-dimension optical imaging system is an extremely complex task. More...