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N-SIM High Resolution Microscope

LS
07/05/2017
Partager
Super-Resolution microscope system offering twice the resolution of conventional optical microscopes

Using an innovative approach based on Structured Illumination Microscopy technology licensed from UCSF, the N-SIM can produce twice the resolution of conventional optical microscopes and a temporal resolution, enabling detailed visualization of minute intracellular structures and their functions.
n-sim

Equipment Configuration

Illumination

  • Lasers : LU-NV series laser unit
    • 405 nm, 50 mW
    • 488 nm, 100 mW
    • 561 nm, 100 mW
    • 640 nm, 100 mW
  • Intensilight external mercury illuminator

Detectors

  • Camera : 2 iXon Ultra DU-897U EMCCD camera mounted on two-camera adapter TuCam (Andor Technology Ltd.)

Objectives

100x CFI HP Apochromat TIRF NA 1.49 WD 0.12mm DIC Oil
60x  CFI SR Plan Apochromat IR  NA 1.27 WD 0.17mm DIC Water
10x CFI Plan Apo Fluor NA 0.45 WD 4mm DIC Dry

Bloc Filters

  • UVA: EX 340-380/DM 400/BA 435-485
  • GFP : EX 465-495/DM 505/BA 515-555
  • TxRed : EX 540-80/DM595/BA 600-660
  • Dual GFP/mCh
  • Cy5 : EX 620/60 /DM660/BA 700/75

Associated Devices

Techniques

  • TIRF-SIM, 2D-SIM, 3D-SIM (Reconstruction method: slice, stack)
  • Lateral resolution (FWHM of beads in xy) ~ 115 nm*1 in 3D-SIM mode, 86 nm*2 in TIRF-SIM mode
  • Axial resolution (FWHM of beads in z)  ~ 269 nm*1 in 3D-SIM mode
  • 3D Axial Range: up to 20μm
  • Live Imaging 2D, 3D and 4D

Applications

  • Cell Biology

Principle

Super resolution Structured Illumination Microscopy

Structured illumination of the excitation light in superresolution microscopy is designed to utilize the moiré effect to obtain finer spatial frequencies emitted by the specimen that can be extracted from Fourier transforms by overlapping two different spatial frequencies from multiple directions. The mathematical transforms create a moiré pattern where the higher spatial frequency information contained in the image can be extracted using software. The result is lateral resolution in the range of 100 nanometers and axial resolution approaching 300 nanometers. More....

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