N-SIM High Resolution Microscope
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Super-Resolution microscope system offering twice the resolution of conventional optical microscopes
Using an innovative approach based on Structured Illumination Microscopy technology licensed from UCSF, the N-SIM can produce twice the resolution of conventional optical microscopes and a temporal resolution, enabling detailed visualization of minute intracellular structures and their functions.
Using an innovative approach based on Structured Illumination Microscopy technology licensed from UCSF, the N-SIM can produce twice the resolution of conventional optical microscopes and a temporal resolution, enabling detailed visualization of minute intracellular structures and their functions.
Nikon Instruments
Equipment Configuration
Illumination
- Lasers : LU-NV series laser unit
- 405 nm, 50 mW
- 488 nm, 100 mW
- 561 nm, 100 mW
- 640 nm, 100 mW
- Intensilight external mercury illuminator
Detectors
- Camera : 2 iXon Ultra DU-897U EMCCD camera mounted on two-camera adapter TuCam (Andor Technology Ltd.)
Objectives
| 100x | CFI HP Apochromat TIRF | NA 1.49 | WD 0.12mm | DIC | Oil |
| 60x | CFI SR Plan Apochromat IR | NA 1.27 | WD 0.17mm | DIC | Water |
| 10x | CFI Plan Apo Fluor | NA 0.45 | WD 4mm | DIC | Dry |
Bloc Filters
- UVA: EX 340-380/DM 400/BA 435-485
- GFP : EX 465-495/DM 505/BA 515-555
- TxRed : EX 540-80/DM595/BA 600-660
- Dual GFP/mCh
- Cy5 : EX 620/60 /DM660/BA 700/75
Associated Devices
- MCL Piezo stage
- Incubator : Stage top incubator for the Nikon N-SIM INUG2H-TIZSH (Tokai Hit Co., Ltd.)
Techniques
- TIRF-SIM, 2D-SIM, 3D-SIM (Reconstruction method: slice, stack)
- Lateral resolution (FWHM of beads in xy) ~ 115 nm*1 in 3D-SIM mode, 86 nm*2 in TIRF-SIM mode
- Axial resolution (FWHM of beads in z) ~ 269 nm*1 in 3D-SIM mode
- 3D Axial Range: up to 20μm
- Live Imaging 2D, 3D and 4D
Applications
- Cell Biology
Principle
Super resolution Structured Illumination Microscopy
Structured illumination of the excitation light in superresolution microscopy is designed to utilize the moiré effect to obtain finer spatial frequencies emitted by the specimen that can be extracted from Fourier transforms by overlapping two different spatial frequencies from multiple directions. The mathematical transforms create a moiré pattern where the higher spatial frequency information contained in the image can be extracted using software. The result is lateral resolution in the range of 100 nanometers and axial resolution approaching 300 nanometers. More....
nsim-mouse-neurons
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