Nikon Imaging Center
Search

Multi colors Upright Microscope

LS
07/03/2017
Partager
The fluorescence microscopy has become an essential tool in biology and the biomedical sciences, as well as in materials science due to attributes that are not readily available in other contrast modes with traditional optical microscopy. The application of an array of fluorochromes has made it possible to identify cells and sub-microscopic cellular components with a high degree of specificity amid non-fluorescing material.
Picture of Eclipse NiE Station at Institut Curie

Equipment Configuration

Illumination

Detectors

Picture of the Eclipse NiE station at Institut Curie

Objectives

with respectively a prism

4x  CFI Plan Fluor  NA 0.13   WD 17.1     Dry  
10x CFI Plan Apo  NA 0.45   WD 4   DIC   Dry  
20x  CFI Plan Apo VC  NA 0.75   WD 1   DIC   Dry  
40x CFI Plan Fluor   NA 1.3   WD 0.2   DIC   Oil  
60x CFI Plan Apo VC  NA 1.4   WD 0.13   DIC   Oil  
100x  CFI Plan Apo VC  NA 1.4   WD 0.13   DIC   Oil  
Picture of the objective Plan Apo VC x100
Picture of a slide with nikon microscope
Picture of the objective Plan Apo VC x100
Picture of a slide with nikon microscope

Bloc Filters

Techniques

Associated Devices

  • Scan slide

Applications

Fix Samples : Immunofluorescence, Histopathology

Principle

Fluorescence Microscopy

The absorption and subsequent re-radiation of light by organic and inorganic specimens is typically the result of well-established physical phenomena described as being either fluorescence or phosphorescence. The emission of light through the fluorescence process is nearly simultaneous with the absorption of the excitation light due to a relatively short time delay between photon absorption and emission, ranging usually less than a microsecond in duration. When emission persists longer after the excitation light has been extinguished, the phenomenon is referred to as phosphorescence. More...

Fluorescent Proteins

The discovery of green fluorescent protein in the early 1960s ultimately heralded a new era in cell biology by enabling investigators to apply molecular cloning methods, fusing the fluorophore moiety to a wide variety of protein and enzyme targets, in order to monitor cellular processes in living systems using optical microscopy and related methodology. When coupled to recent technical advances in widefield fluorescence and confocal microscopy, including ultrafast low light level digital cameras and multitracking laser control systems, the green fluorescent protein and its color-shifted genetic derivatives have demonstrated invaluable service in many thousands of live-cell imaging experiments. More...

color-combine-cell5
color-combine-cell5
cosson
cosson
C-R-p53 2204 D2 normal X10
C-R-p53 2204 D2 normal X10
Histo-Fluo images