Multi colors Upright Microscope
Equipment Configuration
Illumination
- Intensilight precentered fiber mercure lamp
Detectors
- Camera : CCD Camera CoolSNAP HQ2
- Camera : Digital Sight DS-Ri2 Camera
Bloc Filters
Techniques
Associated Devices
-
Scan slide
Applications
Fix Samples : Immunofluorescence, Histopathology
Principle
Fluorescence Microscopy
The absorption and subsequent re-radiation of light by organic and inorganic specimens is typically the result of well-established physical phenomena described as being either fluorescence or phosphorescence. The emission of light through the fluorescence process is nearly simultaneous with the absorption of the excitation light due to a relatively short time delay between photon absorption and emission, ranging usually less than a microsecond in duration. When emission persists longer after the excitation light has been extinguished, the phenomenon is referred to as phosphorescence. More...
Fluorescent Proteins
The discovery of green fluorescent protein in the early 1960s ultimately heralded a new era in cell biology by enabling investigators to apply molecular cloning methods, fusing the fluorophore moiety to a wide variety of protein and enzyme targets, in order to monitor cellular processes in living systems using optical microscopy and related methodology. When coupled to recent technical advances in widefield fluorescence and confocal microscopy, including ultrafast low light level digital cameras and multitracking laser control systems, the green fluorescent protein and its color-shifted genetic derivatives have demonstrated invaluable service in many thousands of live-cell imaging experiments. More...
