Confocal A1r+ Microscope
In the biomedical sciences, a major application of confocal microscopy involves imaging either fixed or living cells and tissues that have usually been labeled with one or more fluorescent probes.
Equipment Configuration
Illumination
- LU-NV series laser unit : 405 nm, 488 nm, 561 nm, 640 nm built-in AOTF
- Intensilight external mercury illuminator
Detectors
- Confocal and Spectral detection in galvano and resonant mode
- Standard fluorescence detector : A1-DUG-2 GaAsP Multi Detector Unit: 2 GaAsP PMTs + 2 Multi-Alkali PMTs
- Spectral detector : A1-DUS spectral detector unit
Objectives
| 10x | CFI Plan Achro | NA 0.25 | WD 10.5 | DIC | Dry |
| 20x | CFI Plan Apo VC | NA 0.75 | WD 1 | DIC | Dry |
| 40x | CFI Plan Fluor | NA 1.3 | WD 0.2 | DIC | Oil |
| 60x | CFI Plan Apo Lambda | NA 1.4 | WD 0.13 | DIC | Oil |
Bloc Filters
- UVA : EX 340-380/DM 400/BA 435-485
- GFP : EX 465-495/DM 505/BA 515-555
- TxRed : EX 540-80/DM595/BA 600-660
- G2A : EX 535-50/DM575/BA 580
Associated Devices
- MCL Piezo stage
- Temperature box and CO2 controler (Life Imaging Service)
Techniques
- Epi-fluorescence
- Laser Scan Microscopy (LSM)
- Förster Resonance Energy Transfer (FRET)
- Fluorescence Recovery After Photobleaching (FRAP)
Applications
- Tissue
- Cell Imaging
Principle
Laser Scanning Confocal Microscopy
Laser scanning confocal microscopes employ a pair of pinhole apertures to limit the specimen focal plane to a confined volume approximately a micron in size. Relatively thick specimens can be imaged in successive volumes by acquiring a series of sections along the optical (z) axis of the microscope.
The confocal Z-Axis Position and widefield Focus sliders are locked together at the same focal plane. They can be uncoupled with the Focus Lock checkbox for observation of a single focal plane in one window while the other is translated through successive view fields along the microscope optical axis. Use either the Z-Axis Position or Focus sliders to scan through successive focal planes, which will produce thin optical sections of the specimen in the confocal image window, and a succession of blurred images in the widefield image window. More...
