Confocal A1r+ Microscope
In the biomedical sciences, a major application of confocal microscopy involves imaging either fixed or living cells and tissues that have usually been labeled with one or more fluorescent probes.
- LU-NV series laser unit : 405 nm, 488 nm, 561 nm, 640 nm built-in AOTF
- Intensilight external mercury illuminator
- Confocal and Spectral detection in galvano and resonant mode
- Standard fluorescence detector : A1-DUG-2 GaAsP Multi Detector Unit: 2 GaAsP PMTs + 2 Multi-Alkali PMTs
- Spectral detector : A1-DUS spectral detector unit
|10x||CFI Plan Achro||NA 0.25||WD 10.5||DIC||Dry|
|20x||CFI Plan Apo VC||NA 0.75||WD 1||DIC||Dry|
|40x||CFI Plan Fluor||NA 1.3||WD 0.2||DIC||Oil|
|60x||CFI Plan Apo Lambda||NA 1.4||WD 0.13||DIC||Oil|
- UVA : EX 340-380/DM 400/BA 435-485
- GFP : EX 465-495/DM 505/BA 515-555
- TxRed : EX 540-80/DM595/BA 600-660
- G2A : EX 535-50/DM575/BA 580
- MCL Piezo stage
- Temperature box and CO2 controler (Life Imaging Service)
- Laser Scan Microscopy (LSM)
- Förster Resonance Energy Transfer (FRET)
- Fluorescence Recovery After Photobleaching (FRAP)
- Cell Imaging
Laser Scanning Confocal Microscopy
Laser scanning confocal microscopes employ a pair of pinhole apertures to limit the specimen focal plane to a confined volume approximately a micron in size. Relatively thick specimens can be imaged in successive volumes by acquiring a series of sections along the optical (z) axis of the microscope.
The confocal Z-Axis Position and widefield Focus sliders are locked together at the same focal plane. They can be uncoupled with the Focus Lock checkbox for observation of a single focal plane in one window while the other is translated through successive view fields along the microscope optical axis. Use either the Z-Axis Position or Focus sliders to scan through successive focal planes, which will produce thin optical sections of the specimen in the confocal image window, and a succession of blurred images in the widefield image window. More...