Nikon Imaging Center
Search

Confocal A1r+ Microscope

LS
07/05/2017
Partager
Confocal microscopy offers several advantages over conventional optical microscopy, including shallow depth of field, elimination of out-of-focus glare, and the ability to collect serial optical sections from thick specimens.
In the biomedical sciences, a major application of confocal microscopy involves imaging either fixed or living cells and tissues that have usually been labeled with one or more fluorescent probes.
ar1-plus

Equipment Configuration

Illumination

  • LU-NV series laser unit : 405 nm, 488 nm, 561 nm, 640 nm  built-in AOTF
  • Intensilight external mercury illuminator

Detectors

  • Confocal and Spectral detection in galvano and resonant mode
    • Standard fluorescence detector : A1-DUG-2 GaAsP Multi Detector Unit: 2 GaAsP PMTs + 2 Multi-Alkali PMTs
    • Spectral detector : A1-DUS spectral detector unit
a1r-plus-station

Objectives

10x CFI Plan Achro NA 0.25   WD 10.5   DIC   Dry  
20x CFI Plan Apo VC  NA 0.75   WD 1   DIC   Dry  
40x CFI Plan Fluor   NA 1.3   WD 0.2   DIC   Oil  
60x CFI Plan Apo Lambda NA 1.4   WD 0.13   DIC   Oil  

Bloc Filters

  • UVA : EX 340-380/DM 400/BA 435-485
  • GFP : EX 465-495/DM 505/BA 515-555
  • TxRed : EX 540-80/DM595/BA 600-660
  • G2A : EX 535-50/DM575/BA 580

Associated Devices

Techniques

  • Epi-fluorescence
  • Laser Scan Microscopy (LSM)
  • Förster Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)

Applications

  • Tissue
  • Cell Imaging

Principle

Laser Scanning Confocal Microscopy

Laser scanning confocal microscopes employ a pair of pinhole apertures to limit the specimen focal plane to a confined volume approximately a micron in size. Relatively thick specimens can be imaged in successive volumes by acquiring a series of sections along the optical (z) axis of the microscope.

The confocal Z-Axis Position and widefield Focus sliders are locked together at the same focal plane. They can be uncoupled with the Focus Lock checkbox for observation of a single focal plane in one window while the other is translated through successive view fields along the microscope optical axis. Use either the Z-Axis Position or Focus sliders to scan through successive focal planes, which will produce thin optical sections of the specimen in the confocal image window, and a succession of blurred images in the widefield image window. More...

 

AZ100-img-1
AZ100-img-1
AZ100-img-2
AZ100-img-2
AZ100-img-3
AZ100-img-3
Confocal Galvano
Confocal Galvano
Images from AZ100 & A1R